Docs CryoEM Sweden
0.0.2

Sample Preparation

  • Overview
  • Carbon Coater
  • Vitrobot
    • Starting
    • Material Preparation
    • Vitrobot Cycle
    • Shutting down
    • Additionaly
  • Graphene Clips
  • Glow Discharge Instructions
    • PELCO easiGlow™
    • Quorum GloQube® Plus

Data Acquisition

  • Grid Preparation
    • Clipping Autogrids
    • Loading of clipped Grids into the Cassette
    • Preparing Nanocap
    • Unloading Existing Cassette
    • Loading of Nanocap into the microscope
    • Loading of Cassette with clipped grids
  • Session Setup
    • Launch the wizard from the command line
    • Setup the parameters in the Session-Wizard GUI
    • Copy raw data to your project folder
      • K2 Camera
      • Falcon 3
    • Next Steps
  • Talos Artica Operation
    • Launch Scipion Session
    • Vacuum and Temperature
    • Cassette Loading and Inventory
    • Start EPU
    • Load EPU Settings
    • Open Column Valves
    • Load cartridge
    • Low Mag Grid Screening
    • Eucentric Height
    • Autofocus
    • Autostigmate
    • Grid Screening
    • Microscope alignment for EPU data collection
    • Check the beam dose
    • Atlas and EPU set up
  • Titan Krios
  • Falcon 3EC
    • Specifications
    • Linear vs counting mode
    • Dose fractioning and frame alignment
    • Exposure and dose-fractionation procedure
      • Example
    • Magnification table, Talso Arctica F3EC, SciLifeLab Solna
    • Magnification table, Glacios F3EC, Uppsala University
    • MTF curves
    • File compression
  • Auto-Functions
    • Eucentric Height
    • Autofocus
    • Autostigmate
    • Autocoma
  • Atlas and EPU
    • Calibrate Image shifts
    • Atlas Acquisition
    • Set up EPU Session
    • Square Selection
    • Hole Selection
  • Advanced Microscopy
    • Condenser Astigmatism
    • Condenser Aperture
    • Beam Tilt Pivot Points
    • Objective Aperture centering
    • Parallel illumination
      • Two vs three condenser lens systems
      • Setting/checking parallel illumination
      • Parallel illumination at gun lens 3, Glacios F3EC, Uppsala University
      • References

Single-Particles

  • Single Particles Analysis

Tomography

  • Tomography

FAQ

  • FAQS
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