Docs CryoEM Sweden
0.0.2
Sample Preparation
Overview
Carbon Coater
Vitrobot
Starting
Material Preparation
Vitrobot Cycle
Shutting down
Additionaly
Graphene Clips
Glow Discharge Instructions
PELCO easiGlow™
Quorum GloQube® Plus
Data Acquisition
Grid Preparation
Clipping Autogrids
Loading of clipped Grids into the Cassette
Preparing Nanocap
Unloading Existing Cassette
Loading of Nanocap into the microscope
Loading of Cassette with clipped grids
Session Setup
Launch the wizard from the command line
Setup the parameters in the Session-Wizard GUI
Copy raw data to your project folder
K2 Camera
Falcon 3
Next Steps
Talos Artica Operation
Launch Scipion Session
Vacuum and Temperature
Cassette Loading and Inventory
Start EPU
Load EPU Settings
Open Column Valves
Load cartridge
Low Mag Grid Screening
Eucentric Height
Autofocus
Autostigmate
Grid Screening
Microscope alignment for EPU data collection
Check the beam dose
Atlas and EPU set up
Titan Krios
Falcon 3EC
Specifications
Linear vs counting mode
Dose fractioning and frame alignment
Exposure and dose-fractionation procedure
Example
Magnification table, Talso Arctica F3EC, SciLifeLab Solna
Magnification table, Glacios F3EC, Uppsala University
MTF curves
File compression
Auto-Functions
Eucentric Height
Autofocus
Autostigmate
Autocoma
Atlas and EPU
Calibrate Image shifts
Atlas Acquisition
Set up EPU Session
Square Selection
Hole Selection
Advanced Microscopy
Condenser Astigmatism
Condenser Aperture
Beam Tilt Pivot Points
Objective Aperture centering
Parallel illumination
Two vs three condenser lens systems
Setting/checking parallel illumination
Parallel illumination at gun lens 3, Glacios F3EC, Uppsala University
References
Single-Particles
Single Particles Analysis
Tomography
Tomography
FAQ
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