Auto-Functions

Eucentric Height

For any imaging or alignment, always bring the speciment to eucentric height (at Grid square magnification).

  1. Centre a grid square.

  2. Go to the auto-functions panel in EPU and select ‘Autoeucentric by stage tilt’, presets grid square, final stage tilt 30 degrees and press start.

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Note

In the status line you will see how the values for z height (Z) and alpha (A) change. It is important that you first centre your feature without tilt and use the z button when the stage is tilted.

Autofocus

  1. Navigate to a carbon area and set the ‘data acquisition’ presets.

  2. Open the direct alignment tab (bottom right of monitor).

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  1. Press Beam shift and centre the beam using multi-function x&y.

  2. Press Done when finished.

Note

If you cannot see the beam lower the magnification.

  1. Go to the auto-functions panel in EPU and select autofunctions-TEM, Autofocus, preset Autofocus

  2. Press Start.

../_images/autofocus_1.PNG

Warning

Do not use the calibrations!

Autostigmate

  1. Go to the auto-functions panel in EPU and select autofunctions-TEM, Autostigmate, preset Thon Ring

  2. Press Start.

../_images/stigmate_1.PNG

Autocoma

  1. Set data acquisition mag.

  2. Go to the auto-functions panel in EPU.

  3. Sselect autofunctions-TEM, Autocoma, preset Thon Ring

  4. Press Start.

../_images/coma.PNG