Docs for the Swedish CryoEM Facility

Here you will find the documentation for

Sample Preparation

• Overview
• Carbon Coater
• Vitrobot
  • Starting
  • Material Preparation
  • Vitrobot Cycle
  • Shutting down
  • Additionaly
• Graphene Clips
• Glow Discharge Instructions
  • PELCO easiGlow™
  • Quorum GloQube® Plus

Data Acquisition

• Grid Preparation
  • Clipping Autogrids
  • Loading of clipped Grids into the Cassette
  • Preparing Nanocap
  • Unloading Existing Cassette
  • Loading of Nanocap into the microscope
  • Loading of Cassette with clipped grids
• Session Setup
  • Launch the wizard from the command line
  • Setup the parameters in the Session-Wizard GUI
  • Copy raw data to your project folder
  • Next Steps
• Talos Artica Operation
  • Launch Scipion Session
  • Vacuum and Temperature
  • Cassette Loading and Inventory
  • Start EPU
  • Load EPU Settings
  • Open Column Valves
  • Load cartridge
  • Low Mag Grid Screening
  • Eucentric Height
  • Autofocus
  • Autostigmate
  • Grid Screening
  • Microscope alignment for EPU data collection
  • Check the beam dose
  • Atlas and EPU set up
• Titan Krios
• Falcon 3EC
  • Specifications
  • Linear vs counting mode
  • Dose fractioning and frame alignment
  • Exposure and dose-fractionation procedure
  • Magnification table, Talso Arctica F3EC, SciLifeLab Solna
  • Magnification table, Glacios F3EC, Uppsala University
  • MTF curves
  • File compression
• Auto-Functions
  • Eucentric Height
  • Autofocus
  • Autostigmate
  • Autocoma
• Atlas and EPU
  • Calibrate Image shifts
  • Atlas Acquisition
  • Set up EPU Session
  • Square Selection
  • Hole Selection
• Advanced Microscopy
  • Condenser Astigmatism
  • Condenser Aperture
  • Beam Tilt Pivot Points
  • Objective Aperture centering
  • Parallel illumination

Single-Particles

• Single Particles Analysis

Tomography

• Tomography

FAQ

• FAQS