Auto-Functions
======================
 
.. _autofunctions-eucentric-height:

Eucentric Height
----------------

For any imaging or alignment, always bring the speciment to eucentric height (at Grid square magnification). 

1. Centre a grid square. 
2. Go to the auto-functions panel in EPU and select 'Autoeucentric by stage tilt', presets grid square, final stage tilt 30 degrees and press start.


.. image:: /images/talos/apertures1.PNG

.. note::

   In the status line you will see how the values for z height (Z) and alpha (A) change. It is important that you first centre your feature without tilt and use the z button when the stage is tilted.
   

Autofocus
---------

1. Navigate to a carbon area and set the 'data acquisition' presets.
2. Open the direct alignment tab (bottom right of monitor).

.. image:: /images/talos/direct_alignments.PNG

3. Press Beam shift and centre the beam using multi-function x&y.
4. Press Done when finished.

.. note::

   If you cannot see the beam lower the magnification.

5. Go to the auto-functions panel in EPU and select autofunctions-TEM, Autofocus, preset Autofocus
6. Press Start.

.. image:: /images/talos/autofocus_1.PNG

.. warning::

    Do not use the calibrations!
    
Autostigmate
------------

1. Go to the auto-functions panel in EPU and select autofunctions-TEM,  Autostigmate, preset Thon Ring
2. Press Start.

.. image:: /images/talos/stigmate_1.PNG


.. _autofunctions-autocoma:

           
Autocoma
--------

1. Set data acquisition mag.
2. Go to the auto-functions panel in EPU.
3. Sselect autofunctions-TEM,  Autocoma, preset Thon Ring
4. Press Start.
	
.. image:: /images/talos/coma.PNG